| 货号 | AF8508-SP |
| 别名 | ARF GAP GIT1; ARF GTPase-activating protein GIT1; CAT1; CAT-1; Cool-associated and tyrosine-phosphorylated protein 1; G protein-coupled receptor kinase interacting ArfGAP 1; G protein-coupled receptor kinase interactor 1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1 | 全称 | G Protein-Coupled Receptor Kinase Interacting ArfGAP 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.025 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human GIT1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.025 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 28964 (Human); 216963 (Mouse); 83709 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human GIT1 Ser485-Asp636 Accession # Q9Y2X7 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | GIT-1 (ARF GTPase-activating protein GIT1) is a 95 kDa protein that belongs to ADP ribosylation factor family and is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration. G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT-1 has also been implicated in neuronal functions including synapse formation and the pathology of Huntington disease. Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains. Htt interacts directly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology. Within amino acids 485-636 human and mouse GIT-1 share 93% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human GIT1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.025 µg/mL of Rabbit Anti-Human GIT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8508) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for GIT1 at approximately 95 & 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
GIT1 in Human Brain. GIT1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Rabbit Anti-Human GIT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8508) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
