| 货号 | AF8480-SP |
| 别名 | AF1P; AF-1P; ALL1 fused gene from chromosome 1; epidermal growth factor receptor pathway substrate 15; epidermal growth factor receptor substrate 15; MLLT5; Protein AF-1p; Protein Eps15 | 全称 | EGFR Pathway Substrate 15 |
| 反应种属 | Human |
| 应用 | Western Blot(0.05 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Eps15 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.05 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2060 (Human); 13858 (Mouse); 313474 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Eps15 Met492-Thr579 Accession # P42566 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | EPS15 (Epidermal growth factor receptor substrate 15) is a 138-140 kDa member of the EPS15 family of proteins. Eps15 has a tripartite structure comprising an amino terminal portion, which contains three evolutionary conserved EH protein-protein interaction domains, a central putative coiled-coil region required for constitutive oligmerization, and a carboxy terminal domain containing multiple copies of the amino acid triplet aspartate-prolinephenylalanine that constitute the AP2 binding domain. The carboxy terminal domain also contains two ubiquitin interaction motifs (UIMs), the last of which is indespensible for Eps15 binding to ubiquitin. Eps15 binds to AP-2 as well as other proteins involved in endocytosis and/or synaptic vesicle recycling, such as synaptojanin1 and epsin. Furthermore, Eps15 colocalizes with markers of the plasma membrane clathrin-coated pits and vesicles.The EPS15 gene yields two isoforms that are believed to reside in distinct subcellular locations and thus implicated in different facets of endosomal trafficking. Human EPS15 has been mapped to chromosome 1p31- p32, a region displaying several non-random chromosomal abnormalities, including deletions in neuroblastoma and translocation in acute lymphoblastic and myeloid leukemias. Over aa 492-579, human EPS15 shares 76% aa identity with mouse EPS15. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Eps15 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Eps15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8480) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Eps15 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Eps15 in HeLa Human Cell Line. Eps15 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Eps15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8480) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to granules in the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Eps15 in NIH‑3T3 Mouse Cell Line. Eps15 was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line using Rabbit Anti-Human Eps15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8480) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
