Human BID Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human BID Affinity Purified Polyclonal Ab (25 UG)

货号： AF846-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF846-SP
别名	apoptic death agonist; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p22 BID
全称	BH3 Interacting Domain Death Agonist
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human BID in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse BID is observed.
使用方法	Western Blot: 1 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	637 (Human); 12122 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TWEAK Induces Apoptosis through a Death-signaling Complex Comprising Receptor-interacting Protein 1 (RIP1), Fas-associated Death Domain (FADD), and Caspase-8. Authors: Ikner A, Ashkenazi A J. Biol. Chem., 2011;286(24):21546-54. Species: Human Sample Type: Cell Lysates Application: WB Enhancement of stress-induced apoptosis in B-lineage cells by caspase-9 inhibitor. Authors: Shah N, Asch RJ, Lysholm AS, Lebien TW Blood, 2004;104(9):2873-8. Species: Human Sample Type: Cell Lysates Application: WB Endoplasmic reticulum calcium pool depletion-induced apoptosis is coupled with activation of the death receptor 5 pathway. Authors: He Q, Lee DI, Rong R, Yu M, Luo X, Klein M, El-Deiry WS, Huang Y, Hussain A, Sheikh MS Oncogene, 2002;21(17):2623-33. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	KLH-coupled human BID synthetic peptide RRELDALGHELPVLAPQWEC
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Bid is a 195 amino acid member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1). Bid is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells Bid is cytosolic. In response to Fas ligand or TNF, Bid is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane (2, 3). Cleavage of Bid by caspase-8 generates a new N-terminous that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target Bid to the mitochondria (4). Bid may then interact withanother pro-apoptotic Bcl-2 family member Bak (5). Interaction of Bid with Bak causes altered mitochondrial membrane permeability. A (9-13) amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the Bid interaction with other Bcl-2 family members. Bid is neutralized by binding to the anti‑apoptotic member Bcl-xL.
运输条件	Blue Ice
存放说明	4℃
参考文献	Gross, A. et al. (1999) Genes and Develop. 13:1899. Luo, X., et al. (1998) Cell 94:481. Li, H. et al. (1998) Cell 94:491. Zha, J. et al. (2000) Science 290:1761. Wei, M.C. et al. (2000) Genes Dev. 14:2060.

参考图片

Detection of Human BID by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 µg/mL anti‑Fas Antibody for 6 hours and WS‑1 human fetal skin fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti‑Human BID Polyclonal Antibody (Catalog # AF846), followed by HRP-conjugated Anti‑Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BID at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.