| 货号 | AF8355-SP |
| 别名 | bA350J20.3; FLJ14735; low density lipoprotein receptor-related protein 11; low-density lipoprotein receptor-related protein 11; LRP-11; MANSC3; MGC39092 | 全称 | LDL Receptor-related Protein 11 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human LRP-11 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 84918 (Human); 237253 (Mouse); 292462 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human LRP-11 Ala38-Gly450 (Pro92Arg) Accession # Q86VZ4 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | LRP11 (Low-density lipoprotein receptor-related protein 11) is a 500 amino acid single-pass type I membrane glycoprotein that exists as 2 alternatively spliced isoforms with predicted molecular weights of 53 and 25 kDa, observed 60 and 35 kDa respectively. LRP11 is a novel member of the LDLR family of proteins. Over aa 38-450, human LRP11 shares 67% aa identity with mouse LRP11. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human LRP-11 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and PANC‑1 human pancreatic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human LRP-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8355) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for LRP-11 at approximately 60 and 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
LRP-11 in Human Brain. LRP-11 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human LRP-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8355) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
