| 货号 | AF8350-SP |
| 别名 | ATP2B2plasma membrane calcium ATPase; ATPase, Ca++ transporting, plasma membrane 4; CALC1; CALCA; Calcitonin 1; CGRP; CT; DKFZp686G08106; DKFZp686M088; EC 3.6.3; EC 3.6.3.8; Katacalcin; Matrix-remodeling-associated protein 1; matrix-remodelling associated 1; MXRA1; PCT; Plasma membrane calcium ATPase isoform 4; Plasma membrane calcium pump isoform 4; plasma membrane calcium pump; plasma membrane calcium-transporting ATPase 4; PMCA4b; PMCA4PMCA4x; Preprocalcitonin; sarcolemmal calcium pump |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Procalcitonin in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 796 (Human); 12310 (Mouse); 24241 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Procalcitonin Ala26-Gly117 (Asn57Asp) Accession # P01258 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human Procalcitonin (CALCA and Calcitonin) is 141 aa in length member of the calcitonin family. Precursor protein is cleaved into a propeptide and the following 2 chains: Calcitonin (aa 85-116) and Katacalcin (aa 121-141). Antigen used to develop this antibody is correspondent to the propeptide and Calcitonin part of the precursor molecule. Secreted Calcitonin is known to cause a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Over aa 26-117, human Procalcitonin shares 80% aa identity with mouse Procalcitonin. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human Procalcitonin by Western Blot. Western blot shows lysates of human thyroid cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Procalcitonin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8350) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Procalcitonin at approximately 15-16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
