| 货号 | AF8301-SP |
| 别名 | APAF-3APAF3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; Cas9; CASP9; CASP-9; caspase 9, apoptosis-related cysteine peptidase; caspase-9; EC 3.4.22.62; ICE-LAP6CASPASE-9c; ICE-like apoptotic protease 6; MCH6apoptosis-related cysteine protease |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Caspase-9 precursor and the pro + large subunit (LSU) that migrates as a 37 kDa band on SDS-PAGE generated by cleavage at Asp330. Does not detect the pro + LSU that migrates as a 34 kDa band on SDS-PAGE generated by cleavage at Asp315. Therefore, the major epitope detected occurs between Asp315 and Asp330. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 842 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Ellipticine derivative NSC 338258 represents a potential new antineoplastic agent for the treatment of multiple myeloma. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Caspase-9 Val139-Asp330 Accession # P55211 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Caspase-9 (Cysteine-aspartic acid protease 9/Casp-9; also APAF-3, Mch6 and ICE-LAP6) is a 35-37 kDa member of the peptidase C14A family of enzymes. Casp-9 is an initiator caspase that is part of the intrinsic apoptosis pathway. It is widely expressed and is particularly important during development. Human proCaspase-9 is a 47-48 kDa, 416 amino acid (aa) protein and it contains one CARD region (aa 1-92) and catalytic residues at His237 and Cys287. Following mitochondrial disruption, cytochrome c is released from mitochrondria. Cytochrome c acts on APAF-1, which induces procaspase-9 dimerization. The act of dimerization activates proCasp-9, leading to either the activation of Casp-3, or the autocleavage of proCasp-9, generating a 35 kDa subunit (aa 1-315) and a 12 kDa subunit. Activated Casp-3 will also act on proCasp-9, generating a 37 kDa subunit (aa 1-330) and a 10 kDa subunit (aa 331-416). These subunits associate to form an active heterotetramer. Casp-9 has an alternative start site at Met84 and a deletion of aa 140-289 that generates a dominant negative, 31 kDa isoform. Over aa 1-134, human Casp-9 shares 81% aa identity with mouse Casp-9; over aa 139-330, human Casp-9 shares 73% aa identity with mouse Casp-9. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Caspase‑9 precursor and pro + large subunit (LSU) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 μg/mL staurosporine (STS) for for the indicated times. PVDF membrane was probed with 1 µg/mL of Human Caspase‑9 Polyclonal Antibody (Catalog # AF8301), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Caspase‑9 precursor and pro + LSU at approximately 46 and 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6. |
