| 货号 | AF8281-SP |
| 别名 | BCL2L3; bcl2-L-3; BCL2L3MGC104264; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; EAT; induced myeloid leukemia cell differentiation protein Mcl-1; Mcl-1; mcl1/EAT; MCL1-ES; MCL1L; MCL1S; MGC1839; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related); TM | 全称 | Myeloid Cell Leukemia Sequence 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human Mcl-1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4170 (Human); 17210 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Mcl-1 Val147-Gly219 Accession # Q07820 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | MCL-1 (induced Myeloid Cell Leukemia differentiation protein 1; also Bcl2L3 and mcl1/EAT) is a 40-45 kDa member of the Bcl-2 family of proteins. It is widely expressed (in B cells, T cells, neutrophils and fibroblasts) and classified as a prosurvival Bcl-2 family member. Functionally, full-length MCL-1 sequesters the proapoptotic proteins Bak and Bax, rendering them inactive. It also delays cell-cycle progression by interacting with CDK1, CHK1 and PCNA. Human MCL-1 is a likely a 350 amino acid (aa) type II transmembrane protein. It contains a large cytoplasmic region (aa 1-327) plus a very short 2 aa C-terminal luminal segment. The cytoplasmic region has multiple domains, including a PEST (Pro/Glu/Ser/Thr)-like segment (aa 104-175), four ubiquitination sites, at least six utilized phosphorylation sites, and three Bcl2-like homology domains (aa 209-223; 252-272; 304-319). MCL-1 is known to form homodimers. There is one splice variant. It is 32-33 kDa in size and contains a 42 aa substitution for aa 230-350. This short form heterodimerizes with full-length MCL-1, rendering it incapable of interacting with Bak and Bax. MCL-1 also undergoes caspase processing. Cleavage after Asp127 generates 17 and 28-30 kDa fragments, while cleavage after Asp 157 generates 21 and 23-25 kDa fragments. These fragments give rise to a proapoptotic environment. Over aa 147-219, human MCL-1 shares 74% aa sequence identity with mouse Mcl-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Mcl‑1 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line and Ramos human Burkitts lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Mcl‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8281) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Mcl‑1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
