| 货号 | AF824-SP |
| 别名 | Apoptosis regulator Bcl-W; BCL2L2; Bcl2-L-2; BCL2-like 2; BCL-W; BCLWbcl-2-like protein 2; KIAA0271BCL2-L-2 | 全称 | B Cell Lymphoma/Leukemia w |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1.2 µg/mL) |
| 目标/特异性 | Detects human and mouse Bcl-w in Western blots. |
| 使用方法 | Western Blot: 1.2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 599 (Human); 12050 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | KLH-coupled human/mouse Bcl-w synthetic peptide Gly32-Asp51 Accession # Q92843 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Bcl-w, also called Bcl-2 like protein 2, is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse Bcl‑w by Western Blot. Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1.2 µg/mL Rabbit Anti-Human/Mouse Bcl‑w Antigen Affinity-purified Polyclonal Antibody (Catalog # AF824) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Bcl‑w Minus C-Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4. |
