| 货号 | AF8247-SP |
| 别名 | amine oxidase [flavin-containing] A; EC 1.4.3; EC 1.4.3.4; MAO-A; monoamine oxidase A; Monoamine oxidase type A | 全称 | Monoamine Oxidase A |
| 反应种属 | Human |
| 应用 | Western Blot(0.25 µg/mL) |
| 目标/特异性 | Detects human MAO-A in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4128 (Human); 17161 (Mouse); 29253 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf21-derived recombinant human MAO-A Met1-Ser497 (predicted) Accession # P21397 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | MAO-A (Monoamine oxidase type A and Amine oxidase [flavin-containing] A) is a 60 kDa (predicted) member of the flavin monoamine oxidase family of proteins. MAO- A is an enzyme that degrades amine neurotransmitters, such as dopamine, norepinephrine, and serotonin. It localizes to the outer mitochondrial membrane. Mutation in this gene results in monoamine oxidase deficiency, or Brunner syndrome, a form of X-linked non-dysmorphic mild mental retardation. Male patients are affected by borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior. Over aa 1-497, human MOA-A shares 88% aa sequence identity with mouse MOA-A. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human MAO-A by Western Blot. Western blot shows lysates of human placenta tissue, human liver tissue, and human kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human MAO-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8247) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MAO-A at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
