| 货号 | AF8246-SP |
| 别名 | Breast cancer-associated protein SGA-1M; MGC10924; N4WBP5; N4WBP5Putative NF-kappa-B-activating protein 164; Nedd4 family interacting protein 1; NEDD4 family-interacting protein 1; NEDD4 WW domain-binding protein 5; Putative MAPK-activating protein PM13; Putative NFKB and MAPK-activating protein | 全称 | Nedd4 Family Interacting Protein 1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(2 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse and rat NDFIP1 in Western blots. |
| 使用方法 | Western Blot: 2 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 80762 (Human); 65113 (Mouse); 291609 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human NDFIP1 Ala10-Asp105 Accession # Q9BT67 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | NDFIP1 (NEDD4 family-interacting protein 1 and NEDD4 WW domain-binding protein 5) is a 26 kDa widelyexpressed protein. NDFIP1 is thought to be part of a family of integral Golgimembrane proteins. It serves as an adaptor for several E3 ubiquitin ligases includingNEDD4 and ITCH, and consequently modulates the stability of their targets.NDFIP1 is genetically linked to human allergic and autoimmune disease, but thecellular mechanism by which these proteins enable foreign and self-antigens tobe tolerated is unresolved. Over aa10-105, human NDFIP1 is 95% identical to mouse andrat NDFIP1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat NDFIP1 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, SK‑Mel‑28 human malignant melanoma cell line, bEnd.3 mouse endothelioma cell line, Hepa 1‑6 mouse hepatoma cell line, C6 rat glioma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat NDFIP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8246) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NDFIP1 at approximately 26-28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
NDFIP1 in SH‑SY5Y Human Cell Line. NDFIP1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Sheep Anti-Human/Mouse/Rat NDFIP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8246) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
