| 货号 | AF8241-SP |
| 别名 | Apoptosis regulator Bcl-W; BCL2L2; Bcl2-L-2; BCL2-like 2; BCL-W; BCLWbcl-2-like protein 2; KIAA0271BCL2-L-2 | 全称 | B Cell Lymphoma/Leukemia w |
| 反应种属 | Human/Mouse |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and mouse Bcl-w. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 599 (Human); 12050 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Bcl-w Ala2-Thr172 Accession # Q92843 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Bcl-w is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
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| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Bcl‑w in Human Spleen. Bcl‑w was detected in immersion fixed paraffin-embedded sections of human spleen using 3 µg/mL Goat Anti-Human/Mouse Bcl‑w Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8241) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
