| 货号 | AF8237-SP |
| 别名 | bA131P10.1; BM-002; C13orf20; chromosome 13 open reading frame 20; ubiquitin-fold modifier 1 | 全称 | Ubiquitin-fold Modifier 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human UFM1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 51569 (Human); 67890 (Mouse); 365797 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human UFM1 Met1-Gly83 Accession # P61960 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | UFM1 ( Ubiquitin-fold modifier 1) is a 9.1 kDaubiquitin-like protein, displaying a similar tertiary structure to ubiquitin.The UFM1 conjugation system is a novel Ubiquitin-like (Ubl) system whosephysiological target(s) and biological functions remain largely undefined. Tobe activated, UFM1 is processed C-terminally by two specific proteases, UfSP1and UfSP2. After processing, UFM1 isactivated via the E1 enzyme, UBA5, and then conjugated by the E2 enzyme, UFC1.UFL1 has been identified as the E3 enzyme. However, cellular functionsassociated with target proteins that are modified by UFM1 are still unknown.Genetic study has demonstrated that the Ufm1-activating enzyme Uba5 isindispensible for erythroid differentiation in mice, highlighting the importanceof this novel system in animal development. It was found that the UFM1 systemwas transcriptionally up-regulated by disturbance of the ER homeostasis andinhibition of vesicle trafficking. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat UFM1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human UFM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8237) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UFM1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
UFM1 in HeLa Human Cell Line. UFM1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human UFM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8237) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
