| 货号 | AF8216-SP |
| 别名 | BFD; CFP; Complement Factor P; complement factor properdin; PFC; PFCcomplement; PFD; PROPERDIN |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat Properdin in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5199 (Human); 18636 (Mouse); 299314 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Complement inhibition by Sarcoptes scabiei protects Streptococcus pyogenes - An in vitro study to unravel the molecular mechanisms behind the poorly understood predilection of S. pyogenes to infect mite-induced skin lesions | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Human embryonic kidney cell line HEK293-derived human Properdin Asp28-Leu469 Accession # P27918 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human Properdin, also known as Complement factor P (CFP), is a 53 kDa secreted glycoprotein that exists inplasma as dimers, trimers or tetramers. It a positive regulator of thealternate pathway of complement. Properdin binds to and stabilizesthe C3- and C5-convertase enzyme complexes and is thought to connect humoraland cellular immunity. Properdin released from activated neutrophils may play amajor role in pattern recognition, whereas plasma properdin may primarilystabilize convertase. Properdin deficiency results in higher susceptibility tobacterial infections. Full-length human Properdin shares 77 % sequence identitywith mouse Properdin. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human, Mouse, and Rat Properdin by Western Blot. Western blot shows lysates of human serum, mouse serum, and rat serum. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Properdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8216) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Properdin at approximately 53-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
