| 货号 | AF819-SP |
| 别名 | BBC2; BBC6; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; BCL2-associated agonist of cell death; Bcl-2-binding component 6; BCL2-binding component 6; BCL2-binding protein; BCL2L8; Bcl2-L-8; BCL2L8bcl2-L-8; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-XL/Bcl-2-associated death promoter | 全称 | Bcl-xL/Bcl-2 Associated Death Promoter |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(0.3-15 µg/mL) |
| 目标/特异性 | Detects human and mouse Bad in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 0.3-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 572 (Human); 12015 (Mouse); 64639 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TCDD promotes lung tumors via attenuation of apoptosis through activation of the Akt and ERK1/2 signaling pathways. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | KLH-coupled human Bad synthetic peptide MFQIPEFEPSEQEDSSSAERGC Accession # Q92934 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Bcl-2 antagonist of cell death (BAD) is an 18 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of BAD on Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Over amino acid 1-21, human BAD shares 81 % aa sequence identity with mouse and rat BAD. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse Bad by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and L‑929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse Bad Antigen Affinity-purified Polyclonal Antibody (Catalog # AF819) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bad at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. | |
Bad in Jurkat Human Cell Line. Bad was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Rabbit Anti-Human/Mouse Bad Antigen Affinity-purified Polyclonal Antibody (Catalog # AF819) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
