| 货号 | AF8176-SP |
| 别名 | antitrypsin), member 7; FLJ17820; member 7; Serpin A7; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); T4-binding globulin; TBG; TBGclade A (alpha-1 antiproteinase; thyroxin-binding globulin; thyroxine-binding globulin |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Serpin A7/TBG in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Serpin A9 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6906 (Human); 331535 (Mouse); 81806 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Serpin A7/TBG Ala21-Ala415 Accession # P05543 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Serpin A7 (also TBG/Thyroxine-binding globulin and T4-binding globulin) is a secreted 54 kDa glycoprotein, non-inhibitory member of the serpin family of proteins. It is expressed by the liver and secreted in plasma. Mutations in the SERPINA7 gene can result in a whole spectrum of deficiencies, characterized by either reduced or increased TBG levels in the serum. A deficiency in TBG result in low serum total T4 and T3 count in clinically euthyroid subjects in the presence of normal serum TSH. Over aa21-415, human serpin A7 shares 76% aa sequence identity with mouse serpin A7. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Serpin A7/TBG by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Serpin A7/TBG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8176) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Serpin A7/TBG at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
