| 货号 | AF8157-SP |
| 别名 | DKFZp686G21125; gp120; H4P; heavy chain-like, 1; IHRP; inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein); inter-alpha-trypsin inhibitor family heavy chain-related protein; inter-alpha-trypsin inhibitor heavy chain H4; ITI-HC4; ITIHL1; PK120; PK-120; plasma kallikrein-sensitive glycoprotein 120; PRO1851 | 全称 | Inter-alpha-Trypsin Inhibitor Heavy Chain H4 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human ITIH4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human ITIH1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3700 (Human); 16427 (Mouse); 54404 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human ITIH4 Glu29-Leu930 (Ile85Asn, Pro698Thr) Accession # Q14624 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The inter-alpha -trypsin inhibitor heavy chain (ITIH4), also known as IHRP (inter-alpha -trypsin heavy chain-related protein), PK-120 (plasma kallikrein-sensitive glycoprotein 120), or Gp120 (glycoprotein 120), is a secreted, 120 kDa, 930 amino acid (aa), N-glycosylated Type II acute phase protein. It is upregulated in response to trauma such as ischemic stroke or myocardial infarction, and functions as a trypsin inhibitor. It is activated by Kallikrein by cleavage between aa 688-689, creating 100 kDa and 35 kDa portions. Mature human ITIH4 shares 66% aa sequence identity with mouse and rat ITIH4. Human isoforms lacking aa 621-650 (914, 900 and 845 aa forms), aa 727-765 and 851-866 (845 aa) and having 14 aa inserted after aa 727 (914 aa) have been described. Proteolytic cleavage by plasma kallikrein produces a 100 kDa fragment that is further processed to 70 kDa, and 35 kDa fragments. An O-glycosylated form is reported in urine, but not in plasma. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human ITIH4 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ITIH4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8157) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ITIH4 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
