| 货号 | AF8156-SP |
| 别名 | homeo box B13; homeobox B13; homeobox protein Hox-B13; PSGD | 全称 | Homeobox B13 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human HOXB13 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10481 (Human); 15408 (Mouse); 303480 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived human HOXB13 Met1-Gln102 Accession # Q92826 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | HOXB13 (Homeobox B13) is a member of the homeobox family of transcription factors with a reported molecular weight of approximately 34 kDa. It is 284 amino acids in length and shares 93% aa identity with mouse and rat HOXB13. HOXB13 plays an important role in prostate cancer. Its expression promotes metastatis of prostate cancers and mutations in HOXB13 are associated with early onset disease. Elevated expression of HOXB13 is also associated with aggressive breast cancers, which may be due in part to its ability to reduce the expression of Estrogen Receptor alpha and increase expression of IL-6. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human HOXB13 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human HOXB13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8156) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HOXB13 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
HOXB13 in LNCaP Human Cell Line. HOXB13 was detected in immersion fixed LNCaP human prostate cancer cell line using Sheep Anti-Human HOXB13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8156) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
HOXB13 in Human Prostate. HOXB13 was detected in formalin fixed paraffin-embedded sections of human prostate using Sheep Anti-Human HOXB13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8156) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
