| 货号 | AF8125-SP |
| 别名 | apo-AIV; apoA-IV; Apolipoprotein A4; apolipoprotein A-IV; MGC142154; MGC142156 |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) |
| 目标/特异性 | Detects human Apolipoprotein A-IV/ApoA in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 337 (Human); 11808 (Mouse); 25080 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-dervied recombinant human Apolipoprotein A-IV/ApoA Glu21-Ser396 Accession # P06727 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Apolipoprotein A4 (ApoA4) is a 45 kDa glycoprotein of the lipid transport system. Secreted in plasma, ApoA4 is a major component of high density lipoprotein (HDL) particles and chylomicrons, and is thought to act in intestinal lipid absorption. Levels of ApoA4 may influence HDL metabolism and modulate its effects on atherogenesis. ApoA4 synthesis in humans is mainly confined to the small intestine, while in mice and rats, production occurs in the liver as well. Human ApoA4 is synthesized as a 396 amino acid (aa) precursor, from which a 20 aa N-terminal signal peptide is removed. Mature human ApoA4 (aa 21-396) shares 61% and 62% aa sequence identity with mouse and rat ApoA4, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Apolipoprotein A‑IV/ApoA4 by Western Blot. Western blot shows human plasma and human serum. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Apolipoprotein A‑IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein A‑IV/ApoA4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
