| 货号 | AF807-SP |
| 别名 | CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3 | 全称 | Urokinase-type Plasminogen Activator Receptor |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunoprecipitation(1 µg/106cells) |
| 目标/特异性 | Detects human uPAR in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse uPAR is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunoprecipitation: 1 µg/106cells Blockade of Receptor-ligand Interaction: In a functional ELISA, 1-5 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) to immobilized Recombinant Human uPAR (Catalog # 807-UK) coated at 5 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5329 (Human); 18793 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Ang II-stimulated migration of vascular smooth muscle cells is dependent on LR11 in mice. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human uPAR Leu23-Arg303 Accession # Q03405 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR cDNA encodes a 335 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, five potential N-linked glycosylation sites and a C‑terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is strictly species-specific. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human uPAR by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and Saos‑2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for uPAR at approximately 30-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
