| 货号 | AF8060-SP |
| 别名 | gastric intrinsic factor (vitamin B synthesis); IF; IFIntrinsic factor; IFMH; IFMHgastric intrinsic factor; INF; TCN3 | 全称 | Gastric Intrinsic Factor |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human GIF in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2694 (Human); 14603 (Mouse); 29319 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Human embryonic kidney cell line HEK293-derived recombinant human GIF Ser19-Tyr417 Accession # P27352 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | GIF (Gastric Intrinsic Factor; also better known as IF or Intrinsic Factor) is a 45-55 kDa, secreted glycoprotein member of the eukaryotic cobalamin transport family of proteins. It should not be confused with either GIF/growth inhibitory factor/metallothionein 3, or GIF/glycosylation-inhibiting factor/macrophage migration inhibitory factor/phenylpyruvate tautomerase. GIF shows restricted expression, being essentially limited to parietal and select chief cells of the stomach. GIF binds to haptocorrin-associated cobalamin/VitB12 in the duodenum, whereupon it passes into the ileum and is taken up by a 500 kDa epithelial receptor named cubam, a complex of cubilin and amnionless molecules. Mature human GIF is 399 amino acids (aa) in length (aa 19-417) that contains one principal B12 binding site between aa 365-395. There is one isoform variant that shows a deletion of aa 3-27. Full-length human GIF shares 82% aa sequence identity with mouse GIF. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human GIF by Western Blot. Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human GIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8060) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GIF at approximately 48-52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
