| 货号 | AF8037-SP |
| 别名 | pigeon homolog (Drosophila); PION; tcag7.1314 | 全称 | Gamma-Secretase Activating Protein |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and mouse GSAP in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 54103 (Human); 212167 (Mouse); 311984 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse GSAP Arg737-Leu858 Accession # Q3TCV3 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | GSAP (GSAP/gamma-Secretase Activating Protein;also known is Pion) is a member of the GSAP family of proteins. It is expressedin neurons, and purportedly serves as a regulator for g-secretase processing ofAPP. Initially, it was thought that cytosolic g-secretase mediated the secondcleavage step in APP processing. In the presence of GSAP, APP (now C99 afterb-secretase cleavage) was preferentially cleaved between either Val40-Ile41, orAla42-Thr43, generating Ab40 and Ab42, respectively. In the absence of GSAP,APP would be preferentially cleaved between L49-Val50. Notably, the presence ofGSAP was shown to have no effect on g-secretase processing of Notch. Subsequentstudies have introduced uncertainty into these relationships. While an absenceof GSAP does apparently reduce Ab production, its presence may not have theregulatory effect once proposed. Mouse GSAP-16K (15-17 kDa) is 121 amino acids (aa) in length(aa 738-858) (SwissProt #:Q3TCV3), it presumably represents a proteolyticcleavage product of the large 95-100 kDa, 858 aa GSAP-FL termed alsoPion/pigeon homolog protein. There are no readily identifiable structuralmotifs associated with the molecule. Mouse PION has two isoform variantsassociated with the gene. One possesses a Phe substitution for aa 172-858,while another possesses a six aa substitution for aa 527-858. Over aa 737-858,mouse GSAP shares 94% and 88% aa sequence identity with rat and human GSAP,respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse GSAP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 1 μg/mL of Sheep Anti-Human/Mouse GSAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8037) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GSAP at approximately 98 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
GSAP in Neuro‑2A Mouse Cell Line. GSAP was detected in immersion fixed Neuro‑2A mouse neuroblastoma cell line using Sheep Anti-Human/Mouse GSAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8037) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
