| 货号 | AF8016-SP |
| 别名 | CGB4; hLHB; interstitial cell stimulating hormone, beta chain; LHB; LSH-B; LSH-beta; luteinizing hormone beta polypeptide; luteinizing hormone beta subunit; lutropin beta chain; Lutropin beta; lutropin subunit beta | 全称 | Luteinizing Hormone beta Polypeptide |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human LH beta in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3972 (Human); 16866 (Mouse); 25329 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human LH beta Ser21-Leu141 Accession # P01229 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | LH beta (Luteinizing Hormone subunit beta; also LSH-B and lutropin beta chain) is a 17-19 kDa member of the glycoprotein hormone subunit beta family, cysteine knot superfamily of proteins. It is synthesized by gonadotrophs in the anterior pituitary, and noncovalently heterodimerizes with the 21-23 kDa common glycoprotein alpha chain, forming 40-42 kDa LH. LH has effects on at least three organ systems. In the ovary, LH induces the synthesis of testosterone, which is quickly converted to estradiol. Estradiol subsequently induces a bolus release of LH from the pituitary, resulting in ovulation. LH now drives the formation of the corpus luteum (or yellow body, from luteus meaning yellow) with the production of progesterone. In the testis, LH induces Leydig cell production of testosterone that activates genes in Sertoli cells drives sperm formation. And in the adrenal, LH appears to induce secretion of sulfated DHEA, a precursor to androgens. Mature human LH beta is 121 amino acids (aa) in length (aa 21-141). It contains three intrachain disulfide bonds that form a cysteine knot, plus one utilized N-linked glycosylation site at Asn50 that incorporates both fucose and sulfate on galactose. The presence of carbohydrate is necessary for bioactivity. Multiple isoforms of LH exist, and likely represent variations in carbohydrate structure. Full-length human LH beta shares 82% and 72% aa sequence identity between human chorionic gonadotrophin/CG beta and mouse LH beta subunits, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human LH beta by Western Blot. Western blot shows lysates of human pituitary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human LH beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8016) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LH beta at approximately 17-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
LH beta in Human Pituitary Adenoma. LH beta was detected in immersion fixed paraffin-embedded sections of human pituitary adenoma using Sheep Anti-Human LH beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8016) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of pituitary cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
