| 货号 | AF8007-SP |
| 别名 | EC 2.7.11; EC 2.7.11.1; GCKR; germinal center kinase-related; KHS1; KHSMEKKK 5; Kinase homologous to SPS1/STE20; MAPK/ERK kinase kinase kinase 5; MAPKKKK5; MEK kinase kinase 5; mitogen-activated protein kinase kinase kinase kinase 5 | 全称 | Hepatocyte Pogenitor Kinase-like/Germinal Center Knase-like Kinase |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human MAP4K5 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11183 (Human); 399510 (Mouse); 503027 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MAP4K5 Lys395-Asn484 (Asn473Lys) Accession # Q9Y4K4 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | MAP4K5 (MAP Kinase Kinase Kinase Kinase 5; also known as KHS, MEKKK5, and GCKR) is a 94-96 kDa member of the STE (STErile 20 protein) subfamily, STE Ser/Thr protein kinase family, protein kinase superfamily of enzymes. It is ubiquitously expressed, and serves as an "intermediary" between GPCRs and cytosolic MAP kinases. It also interacts with Crk (CT10 Regulator of Kinase)-related adaptor molecules, facilitating the formation of signaling complexes around Tyr phosphorylated molecules. And finally, it represents an upstream component of a TNFR1 signaling pathway that requires activation of stress-activated protein kinases. Human MAP4K5 is 846 amino acids 9aa) in length. It contains a 258 aa kinase domain (aa 20-277) that is coupled to a 316 aa CNH (citron homology) domain (aa 506-826) which typically binds GTPases. There are two isoform variants. One shows a deletion of both Pro481 and Glu569, while another possesses a substitution of 16 aa for aa 569-846. Over aa 395-484, human MAP4K5 shares 83% aa sequence identity with mouse MAP4K5. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human MAP4K5 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, HT1080 human fibrosarcoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MAP4K5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8007) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MAP4K5 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
