| 货号 | AF7945-SP |
| 别名 | ETS translocation variant 6; ets variant 6; ets variant gene 6 (TEL oncogene); ETS-related protein Tel1; TEL; TEL/ABL; TEL1; TELTEL1 oncogene; transcription factor ETV6 | 全称 | ETS Translocation Variant 6 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human ETV6 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2120 (Human); 14011 (Mouse); 312777 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Germline ETV6 Mutations Confer Susceptibility to Acute Lymphoblastic Leukemia and Thrombocytopenia. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ETV6 Ser2-Asp111 Accession # P41212 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | ETV6 (ETS Translocation Variant 6; also TEL [Translocated ETS Leukemia]) is a 58-62 kDa member of the ETS (E-Twenty Six avian leukemia virus) family of proteins. It belongs to a particular class of repressors that are controlled by cell-cycle dependent signals. ETV6 is ubiquitously expressed, and acts as a sequence-specific transcriptional repressor of ETS-binding site-containing gene promoters. It likely does this by recruiting histone deacetylase to its binding site. It is also involved in steroid signaling, and is reported to bind to the DNA binding domains of both RAR and RXR. ETV6 appears to be important in coordinating bone marrow-mediated hematopoiesis. Genes known to be responsive to ETV6 regulation include FLI1, IDI and MMP-3. Human ETV6 is 452 amino acids (aa) in length. It contains one PNT (pointed)/B domain that mediates oligomerization (aa 40-124) followed by an ETS domain that binds DNA (aa 338-422). There are five known utilized Thr/Ser phosphorylation sites plus at least one alternative start site at Met43. This short form is very effective at gene repression due to the absence of a SUMOylation site at Lys11 that, when occupied, blocks DNA binding. Although ETV6 activity is dependent upon homooligomerization, it is also known to bind to TEL2 and FLI1. ETS genes are often involved in chromosomal translocations. ETV6 itself is associated with multiple rearrangements, including the genes encoding AML1, PDGFR beta and BTL (Brx-like Translocated in Leukemia). The translocated sequences of ETV6 differ from chimera to chimera. For example, aa 1-336 are fused to a portion of AML1, aa 1-154 are fused to a portion of PDGFR beta, and aa 12-433 are fused to a portion of BTL. Over aa 2-111, human ETV6 shares 91% aa sequence identity with mouse ETV6. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human ETV6 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitts lymphoma cell line. Gels were loaded with 30 µg of cytoplasmic (Cyto) and 15 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ETV6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7945) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ETV6 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
