Human C1qTNF3/CORS26 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human C1qTNF3/CORS26 Affinity Purified Polyclonal Ab (25 UG)

货号： AF7925-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF7925-SP
别名	C1ATNF3; C1q and tumor necrosis factor related protein 3; Cartducin; cartonectin; collagenous repeat-containing sequence of 26-kDa; complement C1q tumor necrosis factor-related protein 3; complement-c1q tumor necrosis factor-related protein 3; Corcs; Cors; CORS26; Cors-26; CTRP3; CTRP32310005P21Rik; FLJ37576; Secretory protein CORS26
全称	C1q and Tumor Necrosis Factor-related Protein 3
反应种属	Human
应用	Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human C1qTNF3/CORS26 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse C1qTNF3/CORS26 is observed.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	114899 (Human); 81799 (Mouse)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human C1qTNF3/CORS26 Asp24-Lys246 Accession # Q9BXJ4
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	C1qTNF3 (Complement C1q TNF-related protein 3/CTRP3; also CORS26 and cartonectin) is a 30-32 kDa, secreted member of the C1q and TNF-related protein (CTRP) superfamily of molecules. It is expressed by a wide variety of cells, including smooth muscle cells, fibroblasts, adipocytes, monocytes and proliferating chondrocytes. C1qTNF3 is an anti-inflammatory agent that apparently blocks LPS activation of mononuclear cells. It also has marked proliferative activity on diverse cell types such as vascular smooth muscle, chrondrocytes, and endothelium. Finally, C1qTNF3 is known to act on hepatocytes and suppress hepatocyte gluconeogenesis. Mature human C1qTNF3 is 224 amino acids (aa) in length (aa 23-246). It possesses an N-terminal collagen-like domain (aa 51-113) followed by a C-terminal globular region (aa 113-246). C1qTNF3 is monomeric when intracellular, but forms a 90 kDa homotrimer plus higher-order oligomer when secreted. There are at least two potential isoform variants. One is 40-42 kDa, glycosylated, and contains a 73 aa insertion after Glu28, while a second shows concurrent deletions of aa 46‑69 and 82-105. The longer 40 kDa isoform is reported to form heterotrimers and oligomers with the standard 30 kDa isoform. This has the effect of protecting the standard isoform from proteolysis. Over aa 24-246, human C1qTNF3 shares 99% aa sequence identity with mouse C1qTNF3.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adipocytokines Complement Pathway Cytokines in Lipid/Cholesterol Metabolism Glucose Homeostasis Obesity

参考图片

Detection of Human C1qTNF3/CORS26 by Western Blot. Western blot shows lysates of human mesenchymal stem cells either undifferentiated or differentiated into adipocytes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human C1qTNF3/
CORS26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7925) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for C1qTNF3/CORS26 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

C1qTNF3/CORS26 in Human Mesenchymal Stem Cells. C1qTNF3/CORS26 was detected in immersion fixed human mesenchymal stem cells differentiated (upper panel) or not (lower panel) into adipocytes using Sheep Anti-Human C1qTNF3/CORS26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7925) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue. Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.