| 货号 | AF7904-SP |
| 别名 | Doc2; DOC2A; doc2-alpha; double C2-like domain-containing protein alpha; double C2-like domains, alpha | 全称 | Double C2-like Domains, alpha |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human and mouse Doc2 alpha in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Doc2 beta is observed. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8448 (Human); 13446 (Mouse); 65031 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Doc2 alpha Met1-Lys114 (Gly48Ser) Accession # Q14183 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | DOC2A/Doc2 alpha (Double C2-like domain containing protein alpha) is a 44 kDa (predicted) monomeric member of the C2 domain-containing protein family of molecules. It is expressed in both neurons and mast cells, and appears to serve as an intracellular Ca++ sensor protein that regulates secretory vesicle release. In neurons, Doc2 alpha is normally bound to synaptic vesicles and interacts with Munc13-1 to promote secretory vesicle exocytosis through the cell membrane. In mast cells, a similar process occurs that involves Munc13-4 instead of Munc13-1. Human Doc2 alpha is 400 amino acids (aa) in length. It contains a Mid domain (aa 13-37) that binds Munc13-1, followed by one C2 domain that binds Ca++ and lipid (aa 91-195), and a second C2 domain that binds SNAP25 (253-356). There is one potential alternative start site 16 aa upstream of the standard site. Over aa 1-114, human Doc2 alpha shares 90% aa sequence identity with mouse Doc2 alpha. Human DOC2B is the product of a separate gene, and shares no meaningful aa sequence identity (< 30%) with human Doc2 alpha. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse Doc2 alpha by Western Blot. Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, P19 mouse embryonal carcinoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, SH‑SY5Y human neuroblastoma cell line, and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Doc2 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7904) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Doc2 alpha at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
