Human SMAC/Diablo Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human SMAC/Diablo Affinity Purified Polyclonal Ab (25 UG)

货号： AF789-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF789-SP
别名	0610041G12Rik; Diablo; diablo, IAP-binding mitochondrial protein; Direct IAP-binding protein with low pI; mitochondrial Smac protein; Second mitochondria-derived activator of caspase; Smac; SMACmitochondrial
全称	Second Mitochondria-derived Activator of Caspase
反应种属	Human
应用	Western Blot(0.5 µg/mL) Simple Western(5 µg/mL)
目标/特异性	Detects human SMAC/Diablo in Western blots. Also detects mouse and rat SMAC/Diablo when the salt concentration of blotting buffer is reduced to 0.15 M NaCl, and 1 μg/mL anti-SMAC/Diablo concentration is used.
使用方法	Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	56616 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies Molecules, 2016;21(8):. Species: Human Sample Type: Whole Cells Application: Flow Proteasome inhibition can impair caspase-8 activation upon submaximal stimulation of apoptotic tumor necrosis factor-related apoptosis inducing ligand (TRAIL) signaling. Authors: Laussmann MA, Passante E, Hellwig CT J. Biol. Chem., 2012;287(18):14402-11. Species: Human Sample Type: Cell Lysates Application: WB Inhibition of lymphotoxin-beta receptor-mediated cell death by survivin-DeltaEx3. Authors: You RI, Chou YC Cancer Res., 2006;66(6):3051-61. Species: Human Sample Type: Whole Cells Application: ICC Role and regulation of nodal/activin receptor-like kinase 7 signaling pathway in the control of ovarian follicular atresia. Authors: Wang H, Jiang JY, Zhu C, Peng C, Tsang BK Mol. Endocrinol., 2006;20(10):2469-82. Species: Rat Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human SMAC/Diablo Ala56-Asp239 Accession # Q9NR28
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	SMAC (second mitochondria derived activator of caspase)/Diablo promotes caspase activation by interacting with the inhibitor of apoptosis (IAP) proteins in the cytochrome c/Apaf-1/caspase-9 pathway.
运输条件	Blue Ice
存放说明	4℃
参考文献	Du, C. et al. (2000) Cell 102:33. Chai, J. et al. (2000) Nature 406:855. Srinivasula, S. et al. (2000) J. Biol. Chem. 275:36152. Ekert, P. et al. (2001) J. Cell Biol. 152:483. Verhagen, A. (2000) Cell 102:43.

参考图片

Detection of Human SMAC/Diablo by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF789) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human SMAC/Diablo (Catalog # 789-SM) (0.5 ng/lane) was included. A specific band for SMAC/Diablo was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

Detection of Human SMAC/Diablo by Simple Western^TM. Simple Western lane view shows lysates of SH‑SY5Y human neuroblastoma cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for SMAC/Diablo at approximately 29 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF789). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.