| 货号 | AF7899-SP |
| 别名 | calcium/calmodulin-dependent protein kinase I; calcium/calmodulin-dependent protein kinase type 1; CaM kinase I alpha; CaM kinase I; CAMKI; caM-KI; caMKI-alpha; EC 2.7.11; EC 2.7.11.17; MGC120317; MGC120318 | 全称 | Calcium/Calmodulin-dependent Protein Kinase I |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects recombinant human CaM Kinase I alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CaMKIV is observed. |
| 使用方法 | Western Blot: 2 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CaM Kinase I alpha Ser176-Arg254 (Ala224Ser) Accession # Q14012 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | CaMKI (Calcium/Calmodulin-dependent kinase I) is a 37-43 kDamonomeric and cytosolic member of the CaMK subfamily, Ser/Thr protein kinasefamily, protein kinase superfamily of molecules. It is found in multiple cell types, includingadrenal cortical cells, neurons and macrophages/osteoclast precursors. CaMKI is associated with the cellular Ca++signaling pathway. Upon entry into thecell, calcium levels are sensed by calmodulin. Upon Ca++ binding, calmodulin (and CaMK kinase) interactswith a Ser/Thr protein kinase termed CaMKIa. CaMKIa is of particular interestin neurons and cells of the zona glomerulosa. In neurons, CaMKIaactivation results in the recruitment of AMPA-Rs and the promotion of axongrowth (vs.dendrite outgrowth which is promoted by CaMKIg). In the adrenal gland, CaMKIa regulates CYP11B2transcription, and thus the ability to synthesize aldosterone. Human CaMKI, the isoform used for immunization, is 370 aminoacids (aa) in length (SwissProt #:Q14012). It contains one protein kinase domain (aa 20-276) followed by acalmodulin-binding region (aa 296-317) and an NES (aa 315-321). Over aa 178-257, human and mouse CaMKI areidentical in amino acid sequence. Thereare three additional CaMKI isoforms (b, g, d), all the product of distinct genes. Nevertheless, over the same aa sequence,these three isoforms (b, g, d)share 80%, 81% and 97% aa sequence identity with CaMKI(a), respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human CaM Kinase I by Western Blot. Western blot shows lysates of IMR‑32 human neuroblastoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human CaM Kinase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7899) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CaM Kinase I at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
CaM Kinase I alpha in Human Hypothalamus. CaM Kinase I was detected in immersion fixed paraffin-embedded sections of human hypothalamus using Sheep Anti-Human CaM Kinase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7899) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
