| 货号 | AF7884-SP |
| 别名 | CMT2A; CMT2A2; CPRP1; CPRP1mitochondrial assembly regulatory factor; EC 3.6.5; EC 3.6.5.-; HSG; KIAA0214hyperplasia suppressor; MARF; MFN2; mitofusin 2; mitofusin-2; Transmembrane GTPase MFN2 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat Mitofusin 2 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human Mitofusin 1 is observed. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9927 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Mitofusin 2 Arg364-Phe599 Accession # O95140 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | MFN-2 (Mitofusin 2; also Hypertension-related protein 1, Mitochondrial assembly regulatory factor, HSG protein, and Transmembrane GTPase MFN-2) is an 85‑100 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in both the ER and outer mitochondrial membrane. Through trans-interactions with MFN-1 and/or MFN-2, MFN-2 apparently mediates the fusion of individual mitochondria with either the ER or with adjacent mitochondria. Pertubations with this process result in both a general failure of mitochondria to fuse, and in cell-specific effects such as a reduction in oxidative phosphorylation, a decrease in the axonal transport of mitochondria, and a deficit in the expression of respiratory chain components. MFN-2 has two key domains. One is a coiled-coil region that mediates MFN-2:MFN-1/2 binding, and a second is a GTPase domain that likely plays a role in fusion. While active, the MFN-2 GTPase domain is 8-fold lower in activity than that for MFN-1. Human MFN-2 is a 757 amino acid (aa) two transmembrane protein that contains a cytoplasmic N- and C‑terminus. The cytoplasmic N‑terminus (aa 1-604) contains the GTPase domain (aa 99-258) while the cytoplasmic C‑terminus possesses a critical coiled-coil motif (aa 696-738). MFN-2 is known to form oligomers, either with itself or MFN-1. Two potential splice variants are reported, one that shows a deletion of aa 245-273, and a second that contains a 33 aa substitution for aa 573-757, suggesting this might be a soluble for of MFN-2. Over aa 364-599, human and mouse MFN-2 share 93% aa sequence identity. Full-length human MFN-2 and MFN-1 share 61% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Mitofusin 2 in Mouse Skeletal Muscle. Mitofusin 2 was detected in perfusion fixed frozen sections of mouse skeletal muscle using Sheep Anti-Human/Mouse/Rat Mitofusin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7884) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
