| 货号 | AF7879-SP |
| 别名 | CFR1; CFR-1; cysteine-rich fibroblast growth factor receptor; DKFZp686L08213; E-selectin ligand 1; ESL1; ESL-1; FLJ23319; FLJ23967; FLJ32797; GLG1; Golgi apparatus protein 1; golgi glycoprotein 1; golgi sialoglycoprotein MG-160; MG160; MG-160 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(2 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Golgi Glycoprotein 1/GLG1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2734 (Human); 20340 (Mouse); 29476 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Golgi Glycoprotein 1/GLG1 Lys1048-Asn1145 Accession # Q92896 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | GLG1 (Golgi complex-Localized Glycoprotein 1; also CFR1, E-Selectin ligand-1/ESL-1, MG-160 and Cys-rich FGF receptor) is a 150-160 kDa (reducing; 130 kDa nonreducing) glycoprotein. It is expressed in both Golgi and/or the cell membrane of multiple cell types, including neutrophils (from rodents; not humans), liver stellate cells, neurons, cardiac myocytes, monocytes and bronchial epithelial cells. In the blood, GLG1/ESL-1 collaborates with PSGL-1 to mediate leukocyte binding to endothelial cell surfaces. PSGL-1 initiates leukocyte tethering while GLG1 promotes slow rolling. GLG1 also serves as an intra-Golgi receptor for multiple FGFs, including FGF-1, -2, -4, -18 and possibly -3, and as a component of an unusual latent TGF-beta complex. Mature human GLG1 is an 1150 amino acid (aa) type I transmembrane protein. It contains a 1116 aa extracellular/luminal region (aa 30-1145) plus a short 13 aa cytoplasmic segment. The extracellular region possesses a 16 aa poly-Gln segment followed by 16 Cys-rich repeats (aa 116-1101). There are three potential isoform variants, one of which possess a 24 aa extension at the C‑terminus, a second that couples the aforementioned C‑terminal extension to a deletion of aa 147-157, and a third that contains a 14 aa substitution for aa 685-1179. It is suggested that the longer C‑terminus retains GLG1 in the Golgi, while shorter cytoplasmic segments allow for presentation at the cell membrane. Over aa 1048‑1145, human and mouse are identical in aa sequence. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Golgi Glycoprotein 1/GLG1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat Golgi Glycoprotein 1/GLG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7879) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Golgi Glycoprotein 1/GLG1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Golgi Glycoprotein 1/GLG1 in PC‑12 Rat Cell Line. Golgi Glycoprotein 1/GLG1 was detected in immersion fixed PC‑12 rat adrenal pheochromocytoma cell line using Sheep Anti-Human/Mouse/Rat Golgi Glycoprotein 1/GLG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7879) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to the Golgi complex. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
