| 货号 | AF7870-SP |
| 别名 | FoxP3-interacting Protein; KIAA1954; NK10; zfp-90; zinc finger protein 476; zinc finger protein 756; zinc finger protein 90 homolog (mouse); zinc finger protein 90 homolog; ZNF756 | 全称 | Zinc Finger Protein 90 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human ZFP90 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse ZFP90 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 146198 (Human); 22751 (Mouse); 498945 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ZFP90 Lys94-Lys207 Accession # Q8TF47 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | ZFP90 (Zinc Finger Protein 90; also ZFP756 and NK10) is a nuclear 66-72 kDa member of the ZNF505 clade, ZNF91 subfamily, kruppel C2H2-type zinc finger family of proteins. It is widely expressed, and found in cell types such as hepatocytes and cardiac myocytes. Functionally, ZFP90 acts as a transcriptional repressor by interacting with KAP-1/TRIM28/TIF1 beta. This interaction influences histone modifications and heterochromatin protein 1 deposition on DNA. Conversely, it is also known to bind to NRSF, thereby promoting NRSF sensitive gene expression. Human ZFP90 is 636 amino acids (aa) in length. It contains one KRAB domain (aa 14-85) followed by thirteen C2H2-type Zn-finger domains (aa 211-631). Over aa 94-207, human and mouse ZFP90 share 54% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human ZFP90 by Western Blot. Western blot shows lysates of IMR‑32 human neuroblastoma cell line and Bowes human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ZFP90 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7870) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZFP90 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
