| 货号 | AF7789-SP |
| 别名 | CMT2B; FLJ20819; member RAS oncogene family; PRO2706; PSN; RAB7; RAB7A, member RAS oncogene family; Ras-associated protein RAB7; ras-related protein Rab-7a | 全称 | RAs Genes from Brain Protein 7a |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and mouse Rab7a in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Rab9a and rhRab9b is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7879 (Human); 19349 (Mouse); 29448 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Rab7a Thr2-Ser204 Accession # P51149 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Rab7a (Ras-related protein Rab 7A; also RAB7) is a 22-24 kDa member of the Rab family, Small GTPase superfamily of proteins. It is widely expressed, and found in cells diverse as skeletal muscle, neurons, osteoclasts and macrophages. Rab7a plays a key role in the formation of phagolysosomes. In particular, Rab7a-GTP becomes associated with early phagosomes. The GTP form of Rab7a first recruits RILP, a molecule that interacts with ORPL1 and promotes the formation of a dynactin:dynein complex. This complex now initiates the movement of the phagosome along microtubules, where it fuses with lysosomes and endosomes at select points, creating phagolysosomes. Human Rab7a is 207 amino acids (aa) in length. It contains multiple Rab family and subfamily motifs, and concludes with a C‑terminal CXC prenylation sequence (aa 205-207). There is one utilized phosphorylation site at Tyr183. Full-length human and mouse Rab7a have identical aa sequences. Notably, however, point mutations with single aa changes are noted in both species. By contrast, human Rab7a and Rab7b share less than 50% aa sequence identity, each representing the product of distinct genes. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human and Mouse Rab7a by Western Blot. Western blot shows lysates of SK‑Mel‑28 human malignant melanoma cell line and B16‑F1 mouse melanoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Rab7a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7789) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Rab7a at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
