| 货号 | AF7780-SP |
| 别名 | dJ955L16.1; DNA-binding protein RFX6; MGC33442; MTFS; Regulatory factor X 6; Regulatory factor X domain-containing protein 1; regulatory factor X, 6; RFXDC1; RFXDC1regulatory factor X domain containing 1 | 全称 | Regulatory Factor X, 6 |
| 反应种属 | Human |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human RFX6 in direct ELISAs and Western blots. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 222546 (Human); 320995 (Mouse); 294395 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human RFX6 Lys324-Thr511 Accession # Q8HWS3 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | RFX6 (regulatory factor X, member 6) is a 928 amino acid, ~102 kDa (calculated) member of the RFX family of transcription factors that are critical for development. RFX6 is expressed in the gut endoderm, later becoming restricted to developing and adult pancreatic islets. It is downstream of Neurogenin-3 and essential for differentiation of most islet cell types. Mutation in humans can produce autosomal recessive neonatal diabetes. Human RFX6 shares 96% and 95% amino acid sequence identity with mouse and rat RFX6, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
RFX6 in Human Pancreas. RFX6 was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human RFX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7780) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
