| 货号 | AF7766-SP |
| 别名 | acid phosphatase 6, lysophosphatidicPACPL1; Acid phosphatase-like protein 1; ACPL1; ACPL1acid phosphatase like 1; EC 3.1.3.2; LPAP; LPAPlysophosphatidic acid phosphatase type 6; PACPL1 | 全称 | Lysophosphatidic Acid Phosphatase 6 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human ACP6 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 51205 (Human); 66659 (Mouse); 295305 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human ACP6 Glu33-Glu428 Accession # BAA91310 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ACP6 (Acid Phosphatase 6, lysophosphatidic; also lysophosphatidic acid phosphatase type 6, LPAP and ACPL1) is a 44-47 kDa monomeric member of the histidine acid phosphatase family of proteins. It is widely expressed, being found in almost all tissues, particularly in mitochondria-rich cells. ACP6 has been described as being both secreted and mitochondrial in location, and plays a critical role in the regulation of lysophosphatidic acid (LPA). LPA is the most structurally simple, biologically active phospholipid in nature (mono-[variable length] acyl-phosphoglycerol). It exists both inside and outside the cell, serving intracellularly as a modulator of lipid rafts, and extracellularly as a signaling molecule that promotes cell growth and fibroblast chemotaxis. ACP6 hydrolyzes LPA, generating monoacylglycerol and phosphate. This presumably eliminates its bioactivity. Human ACP6 precursor is 428 amino acids (aa) in length. It contains a putative signal sequence (aa 1-32) plus a 396 aa mature region (aa 33-428) that possesses one histidine phosphatase domain (aa 120-379). There is one isoform variant that contains an 11 aa substitution for aa 261-428. Mature human ACP6 shares 76% aa sequence identity with mouse ACP6. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human ACP6 by Western Blot. Western blot shows lysates of human prostate tissue and human testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ACP6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7766) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ACP6 at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
ACP6 in Human Ovarian Cancer Tissue. ACP6 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Sheep Anti-Human ACP6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7766) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
