| 货号 | AF7757-SP |
| 别名 | A1B; A1BG; ABG; GAB |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects human alpha 1B-Glycoprotein in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1 (Human); 117586 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Human embryonic kidney cell line HEK293-derived recombinant human alpha 1B-Glycoprotein Ala22-Ser495 Accession # EAW72575 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | A1BG (alpha-1 B/beta-glycoprotein; also C44 [in rodent]) is a 68-80 kDa monomeric plasma glycoprotein member of the Immunoglobulin superfamily of molecules. It is expressed by multiple cell types, principally hepatocytes in response to circulating growth hormone. Initially thought to act as an MMP inhibitor, A1BG is now known to bind to CRISP3, a distant member of a family of venomous molecules. CRISP3 is widely expressed and possesses no toxic properties. But it may play a key role in fertilization, where it temporarily blocks the reaction of PMNs to sperm in the uterus, thus increasing sperm lifespan and the likelihood of fertilization. Within this context, A1BG may regulate CRISP3 availability. A1BG has also been investigated as a biomarker in various cancers. Mature human A1BG is 474 amino acids (aa) in length (aa 22-495) and contains five V-type Ig-like domains. There is one isoform variant that utilizes Met123 as an alternative start site. Full-length human A1BG (aa 22-495) shares only 44% aa sequence identity with mouse A1BG. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human alpha 1B-Glycoprotein by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human alpha 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7757) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha 1B-Glycoprotein at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human alpha 1B-Glycoprotein by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for alpha 1B-Glycoprotein at approximately 94 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human alpha 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7757) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
