| 货号 | AF7747-SP |
| 别名 | Alpha 2,6-ST 2; beta-galactoside alpha-2,6-sialyltransferase 2; beta-galactoside alpha-2,6-sialyltransferase II; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 2; EC 2.4.99; EC 2.4.99.1; FLJ30711; FLJ37730; FLJ38334; hST6Gal II; KIAA1877; KIAA1877St6gal2; sialyltransferase 2 (monosialoganglioside sialyltransferase); Sialyltransferase 2; SIAT2; ST6 beta-galactosamide alpha-2,6-sialyltranferase 2; ST6Gal II; St6GalII | 全称 | ST6 beta-Galactoside alpha-2,6-Sialyltransferase 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human ST6 Gal Sialyltransferase 2/ST6GAL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human ST6GAL1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 84620 (Human); 240119 (Mouse); 301155 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human ST6 Gal Sialyltransferase 2/ST6GAL2 Glu243-Ser529 Accession # Q96JF0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | ST6GAL2 (Sialyltransferase 2; also beta -galactoside alpha -2,6 Sialyltransferase 2) is a 60-62 kDa member of the glycosyltransferase 29 family of enzymes. It has restricted expression, likely being found in neurons and bronchial eipthelium. ST6GAL2 transfers sialic acid from CMP-Neu5Ac to a galactose that is linked to N‑Aceylglucosamine through a beta 1-4 linkage (Gal beta 1-4GlcNac). This transfer will occur when the target motif is found either on select glycoproteins or free oligosaccharides. Notably, the kinetics of this enzyme suggest additional biological functions other than sialic acid transfer. Human ST6GAL2 is a 529 amino acid (aa) type II transmembrane Golgi protein. It contains an 11 aa N‑terminal cytoplasmic region and a 497 aa luminal domain (aa 33-529). The luminal domain possesses an L motif that binds CMP-Sialic acid (aa 296-343), an S motif that binds CMP-Sialic acid and galactose (aa 444-458) and a VS motif that is involved with the enzymes catalytic activity (aa 483-488). There is some evidence that ST6GAL2 may form homodimers. There is one splice variant that shows a 26 aa substitution for aa 441‑529. Over aa 243-529, human ST6GAL2 shares 88% aa sequence identity with mouse ST6GAL2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human ST6 Gal Sialyltransferase 2/ST6GAL2 by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ST6 Gal Sialyltransferase 2/ST6GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7747) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST6 Gal Sialyltransferase 2/ST6GAL2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
ST6 Gal Sialyltransferase 2/ST6GAL2 in Human Brain. ST6 Gal Sialyltransferase 2/ST6GAL2 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human ST6 Gal Sialyltransferase 2/ST6GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7747) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
