| 货号 | AF7746-SP |
| 别名 | CDK6 inhibitor p18; CDKN2C; CDKN6; cyclin-dependent inhibitor; cyclin-dependent kinase 4 inhibitor C; Cyclin-dependent kinase 6 inhibitor; cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4); INK4Ccyclin-dependent kinase 6 inhibitor p18; p18; p18-INK4C; p18-INK6 | 全称 | p18 Cyclin Dependent Kinase 4 Inhibitor 2C |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human p18INK4c/CDKN2C in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human p19INK4d is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1031 (Human); 12580 (Mouse); 54238 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human p18INK4c/CDKN2C Ala2-Gln168 Accession # P42773 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | p18INK4c (18 kDa Inhibitor of CDK4-c; also p18-INK6 and cyclin-dependent kinase 6 inhibitor) is an 18-19 kDa member of the CDKN2 cyclin-dependent kinase inhibitor family of molecules. It is expressed in skeletal muscle, macrophages, T cells and B cells where it serves as a negative regulator of cell proliferation. It does so by specifically associating with either CDK4 or, principally, CDK6, thereby blocking cyclin binding and the activity that leads to a G1-to-S cell cycle transition. In the case of B cells, this activity is necessary for the development of plasma cells. Human p18INK4c is 168 amino acids (aa) in length. It contains four "L" shaped ankyrin repeats (aa 4-132) that interact with cyclin. The C-terminus is described as containing a fifth ankyrin repeat, one that may act to reverse cell cycle repression. Full-length human p18INK4c shares 92% aa sequence identity with mouse p18INK4c. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human p18INK4c/CDKN2C by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human p18INK4c/CDKN2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7746) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p18INK4c/CDKN2C at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
