Human IFN-gamma R2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IFN-gamma R2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF773-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF773-SP
别名	AF-1; AF-1interferon-gamma receptor beta chain precursor10IFNGT1Interferon gamma receptor accessory factor 1; IFGR2; IFN-gamma receptor 2; IFN-gamma-R2; IFNGR2; IFNGT1; interferon gamma receptor 2 (interferon gamma transducer 1); interferon gamma receptor 2; interferon gamma receptor accessory factor-1; interferon gamma receptor beta chain; Interferon gamma transducer 1
全称	Interferon gamma Receptor 2
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human IFN-gamma R2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human IFN‑ gamma R1 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize IFN‑ gamma R2-mediated inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line [Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129]. The Neutralization Dose (ND₅₀) is typically 0.5-2.5 µg/mL in the presence of 2 ng/mL Recombinant Human IFN‑ gamma.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3460 (Human); 15980 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Trisomy 21 consistently activates the interferon response Elife, 2016;5(0):. Species: Human Sample Type: Cell Lysates Application: WB Immunologic analysis of HIV-uninfected Taiwanese children with BCG-induced disease. Authors: Lee WI, Liang FC, Huang JL, Jaing TH, Wang CH, Lin TY, Huang YC, Huang WL, Jou R, Hsieh MY, Chia JH, Wu TL J. Clin. Immunol., 2009;29(3):319-29. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human IFN-gamma R2 Ala22-Gln247 Accession # P38484
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interferon gamma Receptor 2 (IFN-gamma R2) is a type II transmembrane protein that associates with IFN-gamma R1 to form a high affinity receptor complex for IFN-gamma. IFN‑ gamma R2 is required for signal transduction but does not bind IFN-gamma by itself.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Activation Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines and Receptors in Tfh Cell Differentiation and Function IL-10/Interferon Family Receptors in the Jak/STAT Pathway Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th1 Cells Th2 Cells

参考图片

Detection of IFN‑ gamma R2 in human blood‑derived granulocytes

Blood-derived granulocytes were stained with Goat Anti-Human IFN‑ gamma R2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF773, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma R2 Antibody.

Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN‑ gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN‑ gamma R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF773). The ND₅₀ is typically 0.5-2.5 µg/mL.