| 货号 | AF7739-SP |
| 别名 | D0S117E; DBN1; Developmentally-regulated brain protein; DKFZp434D064; Drba; drebrin 1; Drebrin A; Drebrin E; Drebrin E2; drebrin; drebrin-1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Drebrin 1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1627 (Human); 56320 (Mouse); 81653 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Drebrin 1 Asn482-Asp649 (Ser553Pro) Accession # Q16643 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Drebrin 1 (DBN-1 [developmentally-regulated brain protein1]; also drebrin-E/E2 [Embryonic]) is an intracellular member of the ADF-H (actin-depolymerizing factor-H) family of actin binding proteins. Although its predicted MW is 72 kDa, it runs anomalously at 115-116 kDa in SDS-PAGE. It is expressed by neurons, gastric Parietal cells, astrocytes, distal convoluted tubule epithelium and proton-secreting intercalated cells of the renal collecting duct. Drebrin 1 interacts with multiple partners near the membrane. It links connexin-43 and F-actin, thereby stabilizing membrane gap junctions. It also binds to EB3 (end-binding protein 3) on microtubules, facilitating actin-microtubule interactions. Human Drebrin 1 is 649 amino acids (aa) in length. It contains one actin depolymerizing homology domain (aa 3-134), an actin-binding region (≈ aa 150-300), and two HOMER binding motifs (aa 539-543 and 617-621). There are at least 10 utilized Ser/Thr phosphorylation sites and one utilized Tyr phosphorylation site. Alternative splicing generates drebrin-A (Adult), a 124-126 kDa isoform that contains a 46 aa insert after Gly319. Drebrin-A is found in neurons and possibly podocytes, and is associated with dendritic spines where it inhibits the interaction of F-actin with alpha -actinin and tropomyosin. This favors the generation of excitatory impulses in neurons. Three other potential isoform variants are noted. One utilizes an alternative start site at Met64, a second shows a 60 aa substitution for aa 1-110, and a third contains a 28 aa substitution for aa 4-29. Over aa 482-649, human Drebrin 1 shares 84% aa sequence identity with mouse Drebrin 1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human Drebrin 1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Drebrin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7739) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Drebrin 1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Drebrin 1 in HeLa Human Cell Line. Drebrin 1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Drebrin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7739) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
