| 货号 | AF7735-SP |
| 别名 | Deubiquitinating enzyme 8; EC 3.1.2.15; EC 3.4.19.12; HumORF8; KIAA0055hUBPy; MGC129718; ubiquitin carboxyl-terminal hydrolase 8; Ubiquitin isopeptidase Y; ubiquitin specific peptidase 8; ubiquitin specific protease 8; ubiquitin thioesterase 8; Ubiquitin thiolesterase 8; Ubiquitin-specific-processing protease 8; UBPY; UBPYFLJ34456 | 全称 | Ubiquitin Specific Protease 8 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human USP8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human USP2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9101 (Human); 84092 (Mouse); 296121 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human USP8 Met1-Thr1118 Accession # P40818 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | USP8 (Ubiquitin-Specific-Processing protein 8; also UBPY and ubiquitin carboxyterminal hydrolase 8) is a 125-130 kDa member of the peptidase C19 family of proteins. It is a widely expressed deubiquitinating enzyme that regulates the internalization and trafficking of transmembrane growth factor receptors. Ubiquitinated TKRs are typically targeted for internalization and degradation, and USP8 appears to block this activity. Conversely, USP8 has been shown to have opposite effects on GPCR activity. Here, it accelerates ubiquitinated chemokine receptor (CXCR4) internalization and subsequent inactivation, notably through a deubiquitination-independent mechanism. Human USP8 is 1118 amino acids (aa) in length. It contains one MIT domain that mediates endosomal trafficking (aa 33-116), a rhodanese domain that binds RNF proteins (aa 184-305), an SH3 domain binding sequence (aa 405-413), and a C-terminal catalytic domain that hydrolyses ubiquitin adducts (aa 776‑1106). USP8 is phosphorylated at Ser702, 705, 784 and 787. There is one oncogenic recombination isoform that fuses the p85 beta -subunit of PI3 kinase to the C‑terminal 363 aa of USP8, beginning with Pro746. Full-length human and mouse USP8 share 82% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human USP8 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and KG‑1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human USP8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7735) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for USP8 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
