| 货号 | AF7679-SP |
| 别名 | EC 3.5.1.98; HD2; HDAC2; histone deacetylase 2; RPD3; transcriptional regulator homolog RPD3; YAF1; YY1-associated factor 1 | 全称 | Histone Deacetylase 2 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat Histone Deacetylase 2/HDAC2 in Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human HDAC1 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3066 (Human); 15182 (Mouse); 84577 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Histone Deacetylase 2/HDAC2 Pro386-Pro488 Accession # Q92769 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | HDAC2 (Histone deacetylase 2; also HD2) is a 55-60 kDa nuclear member of the class I subfamily, histone deacetylase family of transcriptional regulators. It is found in a variety of cell types including monocytes, cardiac muscle cells and neurons. HDAC2 is also crucial for embryonic development. HDAC2 catalyzes the hydrolytic release of acetyl groups from acetylated histone proteins. Within the context of a large complex, this has the effect of repressing gene activity. Human HDAC2 is 488 amino acids (aa) in length. It contains one histone deacetylation domain (aa 29-388), plus sites for phosphorylation, nitrosylation, ubiquitination, and nitration. There is one alternative start site at Met31. Over aa 386-488, human HDAC2 shares 98% and 53% aa sequence identity with mouse HDAC2 and human HDAC1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human, Mouse, and Rat Histone Deacetylase 2/HDAC2 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Rat‑2 rat embryonic fibroblast cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7679) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
