| 货号 | AF7618-SP |
| 别名 | FLJ36155; GLIS family zinc finger 1; GLI-similar 1; zinc finger protein GLIS1 | 全称 | GLIS Family Zinc Finger 1 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human Glis1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse Glis1 is observed. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 148979 (Human); 230587 (Mouse); 298732 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Glis1 Met446-Thr620 Accession # Q5VTL4 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Glis1 (GLI/glioma-Similar 1; also GliH1) is a 66 kDa (predicted) member of the Kruppel-like zinc finger family of proteins. In the adult, it is principally expressed in placenta and kidney, while in the embryo, it has widespread expression in mesodermal structures. Glis1 can be induced in suprabasal keratinocytes by activating cytokines, and is believed to promote a unique differentiation program. Glis1 is also effective in generating iPSCs from somatic cells (fibroblasts) when concurrently expressed with Oct3, Sox2 and Klf4. Multiple reprogramming pathways are involved, and include Myc, Wnt and Nanog molecules. Functionally, Glis1 is believed to act as a transcriptional repressor. Although it has been described as possessing an activation domain, it would appear that gene promotion is a consequence of the repression of a "repressor". The consequence sequence ACCACCCA corresponds to a GLIS binding motif. Human Glis1 is 620 amino acids (aa) in length. Based on mouse, it contains a zinc-finger repressor region (aa 197-346) plus a putative transactivation domain (aa 448-620). And again based on mouse, there might be an approximately 796 aa splice form that shows an alternative start site 171 aa upstream of the 620 aa isoform. The existence of such an isoform would generate an approximately 95-100 kDa isoform in SDS-PAGE. Over aa 446-620, human Glis1 shares 80% aa sequence identity with mouse Glis1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Glis1 by Western Blot. Western blot shows lysates of HEK001 human epidermal keratinocyte cell line, WS‑1 human fetal skin fibroblast cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, and U‑87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Glis1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7618) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glis1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
