| 货号 | AF7556-SP |
| 别名 | IGFBP3R; IGFBP-3R; Insulin-Like Growth Factor Binding Protein-3 Receptor; Insulin-Like Growth Factor-Binding Protein 3 Receptor; LOC124446; Transmembrane Protein 219 | 全称 | Transmembrane Protein 219 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human TMEM219 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 124446 (Human); 68742 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human TMEM219 Leu41-Leu200 Accession # ACQ72822 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | TMEM219 (Transmembrane protein 219; also IGFBP3 Receptor) is a 30-35 kDa member of the 2-transmembrane group of the Pfam-A family of polytopic molecules. It is widely expressed, and appears to be a cell-surface receptor for IGFBP3. Only free IGFBP3 will bind to TMEM219; when complexed to IGF-I, IGFBP3 is unable to recognize the TMEM219 molecule. Ligation of TMEM219 inhibits cell proliferation and induces caspase-8 mediated apoptosis. It is suggested that procaspase-8 is normally sequestered by TMEM219. Following IGFBP3 binding, procaspase-8 is activated and released from TMEM219, resulting in the downstream activation of executioner caspases. Human TMEM219 is a 240 amino acid (aa) 2-transmembrane protein. It contains an N-terminal 20 aa cytoplasmic region, a 21 aa TM domain, a 164 aa extracellular region (aa 41-204), a second 21 aa TM domain, and a C-terminal 15 aa cytoplasmic tail. Over aa 41-200, human TMEM219 shares 82% aa sequence identity with mouse TMEM219. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TMEM219 by Western Blot. Western blot shows lysates of human liver tissue, human skeletal muscle tissue, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TMEM219 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7556) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TMEM219 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
