| 货号 | AF7528-SP |
| 别名 | EC 2.4.1.41; FLJ93314; GALNACT4; GalNAc-T4; Polypeptide GalNAc transferase 4; polypeptide N-acetylgalactosaminyltransferase 4; pp-GaNTase 4; Protein-UDP acetylgalactosaminyltransferase 4; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4; UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase 4 (GalNAc-T4) | 全称 | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human Polypeptide GalNac Transferase 4/GALNT4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) GALNT1 and rhGALNT3 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8693 (Human); 14426 (Mouse); 500826 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Polypeptide GalNac Transferase 4/GALNT4 Ala36-Lys578 Accession # Q8N4A0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | GALNT4 (Polypeptide N‑acetylgalactosaminyltransferase 4; also Polypeptide GalNAc transferase 4 and UDP-GalNAc:polypeptide Nacetylgalactosaminyltransferase 4) is a 66 kDa member of the GalNAC transferase subfamily, glycosyltransferase 4 family of enzymes. It is widely expressed, highly expressed in mucous cells. GALNT4 is found in the Golgi apparatus, and catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Human GALNT4 is a 578 amino acid (aa) type II transmembrane protein. It contains a twelve aa N-terminal cytoplasmic region and a 543 aa extracellular lumenal domain (aa 36-578). There is a potential 45 kDa splice form variant. Over aa 36-578, human GALNT4 shares 92% aa sequence identity with mouse GALNT4. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human and Mouse Polypeptide GalNac Transferase 4/GALNT4 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Polypeptide GalNac Transferase 4/GALNT4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7528) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Polypeptide GalNac Transferase 4/GALNT4 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
