| 货号 | AF7508-SP |
| 别名 | 2-methylacyl-CoA racemase; alpha-methylacyl-CoA racemase; AMACR; AMACRD; CBAS4; EC 5.1.99.4; RACE; RM |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) |
| 目标/特异性 | Detects human alpha ‑Methylacyl‑CoA Racemase/AMACR in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23600 (Human); 17117 (Mouse); 25284 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human alpha ‑Methylacyl‑CoA Racemase/AMACR Met1-Leu382 Accession # Q9UHK6 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | AMACR (Alpha-MethylAcyl-CoA Racemase; also 2-methylacyl racemase) is a 43-46 kDa member of the CaiB/BaiF CoA-transferase family of enzymes. It is widely expressed, being found in fibroblasts, hepatocytes, plus tumorigenic prostatic and colonic epithelium. Within these cells, it is localized to peroxisomes (organelles that participate in the breakdown fatty acids into 2-carbon blocks) and occasionally mitochondria, and appears to racemize 2-methyl-branched fatty acids. This ability is necessary for the degradation of branched fatty acids such as C19 dietary pristanic acid. Pristanic acid occurs in both an S- and R-methylated stereoisomer, but can only be initially degraded in the S- isomeric form. AMACR converts the R- to the S-isoform, initiating fatty acid processing. Human AMACR(-IA) is 382 amino acids (aa) in length. It contains an N-terminal mitochondrial targeting sequence (aa 22-85) that overlaps the enzymatic region (aa 53-231), and a C-terminal peroxisomal targeting motif (aa 379-382). There are multiple potential splice variants. Over aa 132-382, there are three aa substitutions, one that is 66 aa in length (AMACR‑IB), a second that is 147 aa in length (AMACR-IIB), and a third that is 98 aa in length. Over aa 249-382, there are two aa substitutions, one that is 13 aa in length (AMACR‑IIAs), and another that is 41 aa in length (AMACR-IIA). There is also a sixth potential splice variant that shows a 16 aa substitution for aa 378-382. Full-length human AMACR(-IA) shares 77% aa sequence identity with mouse AMACR. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human alpha ‑Methylacyl‑CoA Racemase/AMACR by Western Blot. Western blot shows lysates of human kidney tissue, human liver tissue, and human prostate tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human alpha ‑Methylacyl‑CoA Racemase/AMACR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7508) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha ‑Methylacyl‑CoA Racemase/AMACR at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
