| 货号 | AF7497-SP |
| 别名 | 18 kDa cationic antimicrobial protein; CAMP; CAP18; CAP18CRAMP; cathelicidin antimicrobial peptide; CRAMP; FALL39; FALL-39; FALL39CAP-18; hCAP-18; LL37 | 全称 | Cathelicidin Antimicrobial Peptide |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human CAMP/LL37/FALL39 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 820 (Human); 12796 (Mouse); 316010 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CAMP/LL37/FALL39 Leu134-Ser170 Accession # P49913 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | CAMP (Cathelicidin AntiMicrobial Peptide; also18 kDa cationic antimicrobial protein, CAP18, LL37, FALL39 and HSD26) is a member of the cathelicidin family of proteins. It is widely expressed, being found associated with neutrophils, bronchial epithelium, renal tubule epithelium, activated keratinocytes, gamma δ T cells, monocytes, NK cells, colonic epithelium and the stratum basale of nonkeratinized epithelium found in the vagina and oral cavity. CAMP has marked antimicrobial activity against both Gm+ and Gm- bacteria, and acts as a chemoattractant for neutrophils, monocytes and mast cells. CAMP is synthesized as 170 amino acid (aa) preproprecursor. It contains a 30 aa signal sequence, a 103 aa, 14 kDa prosegment (aa 31-131), and a 4-5 kDa, 37 aa (aa 134-170) C‑terminal mature fragment (LL37) or 39 aa (aa 132‑170) C‑terminal mature fragment (FALL39). In neutrophils, the 18-19 kDa proprecursor is stored in granules, where, upon activation, it is enzymatically cleaved and released. While both the prosegment and C‑terminal fragments possess antimicrobial activity, the prosegment also shows antiprotease activity, while the C‑terminal fragment also shows chemotactic activity. The prosegment may form homodimers, while the C‑terminal fragment (LL37) is reported to form homotetramers. Over aa 141-170, human CAMP shares only 50% and 57% aa sequence identity with mouse and rat CAMP, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human CAMP/LL37/FALL39 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CAMP/LL37/FALL39 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7497) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CAMP at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
