| 货号 | AF7458-SP |
| 别名 | cytosolic, 1C, member 1; EC 2.8.2; EC 2.8.2.-; EC 2.8.2.1; EC 2.8.2.2; humSULTC2; ST1C1; ST1C2; Sulfotransferase 1C1; sulfotransferase family, cytosolic, 1C, member 2; SULT1C#1; SULT1C1; SULT1C2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Cytosolic Sulfotransferase 1C2/SULT1C2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SULT1C4 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6819 (Human); 316153 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Cytosolic Sulfotransferase 1C2/SULT1C2 Ala2-Leu296, predicted Accession # O00338 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Cytosolic sulfotransferases catalyze the sulfonation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi-resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm (1, 2). SULT1C2 is mainly expressed in the gastrointestinal tract (stomach, duodenum, jejunum, ileum, colon, caecum and rectum), liver and kidneys, but not in the lungs (3). In contrast, SULT1C4, a sulfotransferase that is most closely related to SULT1C2 (4), is expressed at higher levels in fetal lung and kidney and at lower levels in fetal heart. So far, SULT1C2 is found to be active only on p-nitrophenol (3). The enzymatic activity of our recombinant human SULT1C2 was determined using a phosphatase-coupled assay (5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Cytosolic Sulfotransferase 1C2/SULT1C2 by Western Blot. Western blot shows lysates of Caki‑2 human clear cell carcinoma epithelial cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Cytosolic Sulfotransferase 1C2/ SULT1C2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7458) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytosolic Sulfotransferase 1C2/SULT1C2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
