| 货号 | AF7440-SP |
| 别名 | DSHP; DSHPLYP; Duncan disease SH2-protein; EBVS; FLJ92177; IMD5; LYP; MTCP1; SAP/SH2D1A; SAPlymphoproliferative syndrome; SH2 domain containing 1A; SH2 domain-containing protein 1A; signaling lymphocyte activation molecule-associated protein; Signaling lymphocytic activation molecule-associated protein; SLAM associated protein/SH2 domain protein 1A; SLAM-associated protein; T cell signal transduction molecule SAP; T-cell signal transduction molecule SAP; XLP; XLPD; XLPDFLJ18687; XLPSH2 domain protein 1A |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human SH2D1A in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4068 (Human); 20400 (Mouse); 501502 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human SH2D1A Met1-Pro128 Accession # O60880 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | SH2D1A (Src-Homology 2 Domain-Containing protein A1; also SAP and DSHP) is a 15-19 kDa protein that represents the product of the XLP (X-linked Lymphoproliferative Disease) gene. It is a cytoplasmic protein that is expressed in T cells, NK cells, and memory B cells. SH2D1A interacts with the SH2 binding domains of at least six SLAM family cell membrane proteins, including Ly9, CD84, 2B4/CD244, NTBA, CS1 and SLAM/CD150. This has the effect of blocking an SHP2 interaction with the above phosphorylated receptors and abrogating downstream signaling. Notably, SH2D1A also recruits and activates the Src kinase Fyn when bound to SLAM family molecules, and this action presumably impacts additional downstream signaling. Human SH2D1A is 128 amino acids (aa) in length. Although it contains only one SH2 domain (aa 6-104), this domain can be utilized simultaneously by SLAM and Fyn, creating a trimeric complex. There are multiple splice variants for SH2D1A. One shows a three aa deletion of aa 114-116, a second possesses a nine aa substitution for aa 68-128, a third contains a 16 aa substitution for aa 47-128, a fourth possesses a 12 aa substitution for aa 47-128, while a fifth contains a 37 aa substitution for aa 40-128. Full length human SH2D1A shares 88% aa identity with mouse SH2D1A. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human SH2D1A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SH2D1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7440) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SH2D1A at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 |
