| 货号 | AF7417-SP |
| 别名 | DFNA14; DFNA38; DFNA6; DIDMOAD; FLJ51211; WFRS; WFSwolframin; Wolfram syndrome 1 (wolframin); Wolframin | 全称 | Wolfram Syndrome 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human WFS1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7466 (Human); 22393 (Mouse); 83725 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human WFS1 Lys679-Phe783 Accession # O76024 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | WFS1 (Wolframin Syndrome gene 1; also Wolframin) is a 100-105 kDa intracellular glycoprotein that contains an unusual eleven transmembrane (TM) topology. It is widely expressed, being found in neurons, fibroblasts, hepatocytes, stratified squamous epithelium and pancreatic beta -cells. WFS1 is found in the ER and select secretory vesicles. It is known to be induced by ER stress, which prompts it to increase Ca++ in the ER, a condition necessary for proper protein folding. It also contributes to the maintenance of the proper pH in insulin-containing granules. Human WSF1 is 890 amino acids (aa) in length. It is a type II 11-TM protein that possesses a cytoplasmic N-terminus (aa 1-313) and transmembrane-embedded C-terminus (aa 870-890). WSF1 is reported to form homodimers and homotetramers. There are multiple mutations in the WFS1 gene that contribute to Wolfram syndrome. Among these are an isoform that generates a premature truncation at Ser157, a second isoform that possesses a seven aa substitution for aa 509-890, and a third isoform which shows a deletion of aa 508-512. Over aa 679-783, human WFS1 shares 95% aa sequence identity with mouse WFS1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human WFS1 by Western Blot. Western blot shows lysates of IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human WFS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7417) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for WFS1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
