| 货号 | AF7366-SP |
| 别名 | Myobrevin; VAMP-5; vesicle-associated membrane protein 5 (myobrevin); vesicle-associated membrane protein 5 | 全称 | Vesicle-Associated Membrane Protein 5 |
| 反应种属 | Human |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human VAMP-5 in direct ELISAs. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) VAMP-7 is observed, and less than 1% cross-reactivity with rhVAMP-1, rhVAMP-2, and rhVAMP-8 is observed. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10791 (Human); 53620 (Mouse); 89818 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human VAMP-5 Ala2-Cys72 Accession # O95183 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | VAMP-5 (vesicle associated membrane protein 5; also myobrevin) is a member of the synaptobrevin family of proteins. Although its predicted MW is 11 kDa, it runs anomalously at 16 kDa in SDS-Page. VAMP-5 is a type IV transmembrane (TM) protein (i.e.-a type II TM protein whose C-terminus is almost completely transmembrane) that is found in both trans-Golgi and plasma membranes of myotubes (mature skeletal and cardiac muscle cells). While VAMPs are commonly associated with vesicle fusion to t-SNARE in cell membranes, VAMP-5 appears to show no such activity. Human VAMP-5 is 116 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-72) with one vSNARE coiled-coil homology domain, a 21 aa TM segment, and 23 aa C-terminal luminal domain. Over aa 1-72, human VAMP-5 shares 75% aa identity with mouse VAMP-5. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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VAMP-5 in Human Skeletal Muscle. VAMP-5 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Sheep Anti-Human VAMP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7366) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
